Skip to main content
Addgene

pBiFCt-2in1-NC
(Plasmid #105112)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 105112 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pBiFCt-2in1-NC
  • Vector type
    Plant Expression
  • Selectable markers
    lacZ
  • Tags / Fusion Proteins
    • nYFP-HA (N terminal on backbone)
    • cYFP-Myc (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Spectinomycin, 25 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Tags / Fusion Proteins
    • nYFP-HA (N terminal on backbone)
    • cYFP-Myc (C terminal on backbone)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBiFCt-2in1-NC was a gift from Christopher Grefen (Addgene plasmid # 105112 ; http://n2t.net/addgene:105112 ; RRID:Addgene_105112)

  • For your References section:

    A 2in1 cloning system enables ratiometric bimolecular fluorescence complementation (rBiFC). Grefen C, Blatt MR. Biotechniques. 2012 Oct 2;53(5):311-14. doi: 10.2144/000113941. 10.2144/000113941 PubMed 23066669
Included in kit:
Related items:
Commonly requested with: