pHACK-GAL4>QF2(newHA1)
(Plasmid #104873)

• Purpose: HACK Donor plasmids for converting GAL4 lines to QF2 lines using HACK method. Updated version of Addgene#80275 for easier cloning of inserts
• Depositing Lab: Christopher Potter
• Publication: Lin et al Genetics. 2016 Jun 22. pii: genetics.116.191783.

Backbone

• Vector backbone: pHACK
• Backbone manufacturer: Potter Lab
• Backbone size w/o insert (bp): 4967
• Vector type: Insect Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: T2A-QF2
• Alt name: gRNA1 against GAL4
• Alt name: gRNA2 against GAL4
• Species: S. cerevisiae (budding yeast), Synthetic
• Insert Size (bp): 5194

Cloning Information

• Cloning method: Ligation Independent Cloning

Resource Information

• Supplemental Documents:
  • pHACK-GAL4-_QF2(newHA1).gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This is an updated version of pHACK-GAL4>QF2 (Addgene#80275) which introduces AvrII/SphI restriction sites to easily excise QF2-hsp70 for cloning of other gene cassettes into a GAL4 HACK construct.