pCDH-EF1a-extGluc-mKate-NGFR
(Plasmid
#104831)
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PurposeextGluc luciferase for invivo Imaging, mKate and NGFR for flourescent detection
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 104831 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCDH-EF1a-MCS-T2A-copGFP
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Backbone manufacturerSystem Biosciences
- Backbone size w/o insert (bp) 7260
- Total vector size (bp) 8887
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Vector typeMammalian Expression, Lentiviral
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Selectable markersmKate; NGFR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert namemKate
Gene/Insert 2
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Gene/Insert nameNGFR
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDH-EF1a-extGluc-mKate-NGFR was a gift from Irmela Jeremias (Addgene plasmid # 104831 ; http://n2t.net/addgene:104831 ; RRID:Addgene_104831) -
For your References section:
Characterization of Rare, Dormant, and Therapy-Resistant Cells in Acute Lymphoblastic Leukemia. Ebinger S, Ozdemir EZ, Ziegenhain C, Tiedt S, Castro Alves C, Grunert M, Dworzak M, Lutz C, Turati VA, Enver T, Horny HP, Sotlar K, Parekh S, Spiekermann K, Hiddemann W, Schepers A, Polzer B, Kirsch S, Hoffmann M, Knapp B, Hasenauer J, Pfeifer H, Panzer-Grumayer R, Enard W, Gires O, Jeremias I. Cancer Cell. 2016 Dec 12;30(6):849-862. doi: 10.1016/j.ccell.2016.11.002. Epub 2016 Dec 1. 10.1016/j.ccell.2016.11.002 PubMed 27916615