pcDNA-Flag-Kdm4b-pA
(Plasmid
#104486)
-
PurposeExpress mouse Kdm4b in mammalian cells. It has a T7 promoter for in vitro transcription. Since polyA is encoded on the vector, polyA-tailing is unnecessary.
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 104486 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepcDNA3.1
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameKdm4b
-
Alt nameJmjd2b
-
SpeciesM. musculus (mouse)
-
Entrez GeneKdm4b (a.k.a. 4732474L06Rik, Jmjd2b)
-
Tag
/ Fusion Protein
- Flag epitope tag (N terminal on insert)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pcDNA-Flag-Kdm4b-pA was a gift from Yi Zhang (Addgene plasmid # 104486 ; http://n2t.net/addgene:104486 ; RRID:Addgene_104486) -
For your References section:
Genomic imprinting of Xist by maternal H3K27me3. Inoue A, Jiang L, Lu F, Zhang Y. Genes Dev. 2017 Oct 1;31(19):1927-1932. doi: 10.1101/gad.304113.117. 10.1101/gad.304113.117 PubMed 29089420
