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Addgene

pcDNA3.1/Hygro(-)-B2-cT
(Plasmid #104355)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 104355 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1/Hygro(-)
  • Backbone size w/o insert (bp) 5596
  • Total vector size (bp) 9385
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Human integrin b2 with tension sensor
  • Alt name
    ITGB2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4194
  • Entrez Gene
    ITGB2 (a.k.a. CD18, LAD, LCAMB, LFA-1, MAC-1, MF17, MFI7)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)
  • 5′ sequencing primer CMV_fwd
  • 3′ sequencing primer BGH_rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1/Hygro(-)-B2-cT was a gift from Pontus Nordenfelt & Timothy Springer (Addgene plasmid # 104355 ; http://n2t.net/addgene:104355 ; RRID:Addgene_104355)

  • For your References section:

    Coordinated integrin activation by actin-dependent force during T-cell migration. Nordenfelt P, Elliott HL, Springer TA. Nat Commun. 2016 Oct 10;7:13119. doi: 10.1038/ncomms13119. 10.1038/ncomms13119 PubMed 27721490
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