mGarnet-alpha-tubulin
(Plasmid
#104317)
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Purposecodes for a fusion of fluorescent protein mGarnet and alpha-tubulin, visualizes alpha-tubulin
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 104317 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEGFP
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemGarnet-alpha-tubulin
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Insert Size (bp)2082
- Promoter CMV
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mGarnet-alpha-tubulin was a gift from Ulrich Nienhaus (Addgene plasmid # 104317 ; http://n2t.net/addgene:104317 ; RRID:Addgene_104317) -
For your References section:
Monomeric Garnet, a far-red fluorescent protein for live-cell STED imaging. Hense A, Prunsche B, Gao P, Ishitsuka Y, Nienhaus K, Nienhaus GU. Sci Rep. 2015 Dec 9;5:18006. doi: 10.1038/srep18006. 10.1038/srep18006 PubMed 26648024
