GST-AR-1-566
(Plasmid #104195)

• Purpose: Expresses human AR-1-566 from bacterial expression vector pGEX-5X-1 as GST fusion
• Depositing Lab: Elizabeth Wilson
• Publication: He et al J Biol Chem. 2000 Jul 28;275(30):22986-94.

Backbone

• Vector backbone: pGEX-5x-1
• Backbone manufacturer: Pharmacia
• Backbone size w/o insert (bp): 4900
• Total vector size (bp): 6500
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL-1 blue
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: human androgen receptor aa 1-566
• Species: H. sapiens (human)
• Insert Size (bp): 1600
• GenBank ID: AAA51886.1
• Entrez Gene: AR (a.k.a. AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH1 (not destroyed)
• 3′ cloning site: EcoR1 (destroyed during cloning)
• 5′ sequencing primer: don't have
• 3′ sequencing primer: don't have

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The GST-AR insert has an insertion of 3 glutamine residues at amino acid 74 and a deletion of six amino acids (GGGGGE) after amino acid 466 compared to the listed reference sequence. The depositor has confirmed that this should not affect plasmid function.