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Addgene

p3s-Cas9-HF1
(Plasmid #104173)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 104173 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p3s
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas9-HF1
  • Mutation
    N497A, R661A, Q695A, Q926A
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p3s-Cas9-HF1 was a gift from Jin-Soo Kim (Addgene plasmid # 104173 ; http://n2t.net/addgene:104173 ; RRID:Addgene_104173)

  • For your References section:

    Rescue of high-specificity Cas9 variants using sgRNAs with matched 5' nucleotides. Kim S, Bae T, Hwang J, Kim JS. Genome Biol. 2017 Nov 15;18(1):218. doi: 10.1186/s13059-017-1355-3. 10.1186/s13059-017-1355-3 [pii] PubMed 29141659
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