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Addgene

pGL3-BASIC- ARHGAP29 Mutant PROMOTER
(Plasmid #104156)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 104156 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL3 basic
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ARHGAP29 promoter
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1500
  • Mutation
    delete two TEAD binding sites
  • Entrez Gene
    ARHGAP29 (a.k.a. PARG1)
  • Promoter ARHGAP29
  • Tag / Fusion Protein
    • luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)
  • 5′ sequencing primer RVprimer3
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL3-BASIC- ARHGAP29 Mutant PROMOTER was a gift from Marius Sudol (Addgene plasmid # 104156 ; http://n2t.net/addgene:104156 ; RRID:Addgene_104156)

  • For your References section:

    YAP Regulates Actin Dynamics through ARHGAP29 and Promotes Metastasis. Qiao Y, Chen J, Lim YB, Finch-Edmondson ML, Seshachalam VP, Qin L, Jiang T, Low BC, Singh H, Lim CT, Sudol M. Cell Rep. 2017 May 23;19(8):1495-1502. doi: 10.1016/j.celrep.2017.04.075. 10.1016/j.celrep.2017.04.075 PubMed 28538170
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