pBS-TRE-EYFP-WPRE
(Plasmid #104104)

• Purpose: Expresses EYFP under the TRE promoter in mammalian cells.
• Depositing Lab: Takeshi Imai
• Publication: Sakaguchi et al Elife. 2018 Nov 20;7. pii: 40350. doi: 10.7554/eLife.40350.

Backbone

• Vector backbone: pBluescript SK(+)
• Backbone manufacturer: Stratagene
• Modifications to backbone: An Xho-Xho fragment containing TRE-SV40 poly A was transferred from pTRE-Tight (Clontech).
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EYFP
• Species: Aequorea victoria
• Insert Size (bp): 720
• Promoter: TRE

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (unknown if destroyed)
• 3′ cloning site: NotI (unknown if destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• Supplemental Documents:
  • pBS-TRE-EYFP-WPRE.str
• A portion of this plasmid was derived from a plasmid made by: The EYFP gene was cloned from pEYFP-N1 from Clontech.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry