eGFP-KCC2
(Plasmid #104075)

• Purpose: expresses rat KCC2 (b isoform) with eGFP tag at the N-terminus of KCC2
• Depositing Lab: Igor Medyna
• Publication: Pellegrino et al J Physiol. 2011 May 15;589(Pt 10):2475-96. doi: 10.1113/jphysiol.2010.203703. Epub 2011 Mar 21.

Backbone

• Vector backbone: EGFP-C1
• Backbone size w/o insert (bp): 4731
• Total vector size (bp): 8157
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: KCC2 (b isoform)
• Alt name: Slc12a5
• Species: R. norvegicus (rat)
• Insert Size (bp): 3350
• Mutation: EcoR1 site in rat KCC2 was removed by silent mutation, A3267G in rat coding sequence
• Entrez Gene: Slc12a5 (a.k.a. Kcc2)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Bgl II (unknown if destroyed)
• 3′ cloning site: EcoR1 (unknown if destroyed)
• 5′ sequencing primer: EGFP-C . CATGGTCCTGCTGGAGTTCGTG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry