aLL-EGFP
(Plasmid #103880)

• Purpose: aL-EGFP with 4aa linker
• Depositing Lab: Timothy Springer
• Publication: Nordenfelt et al Nat Commun. 2017 Dec 11;8(1):2047. doi: 10.1038/s41467-017-01848-y.

Backbone

• Vector backbone: pcDNA3.1/hygro(-)
• Backbone size w/o insert (bp): 5400
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: full-length human aL with EGFP in betapropeller
• Alt name: aL-EGFP with 4aa linker
• Alt name: aLL-EGFP
• Alt name: integrin subunit alpha L
• Species: H. sapiens (human)
• Insert Size (bp): 4300
• Entrez Gene: ITGAL (a.k.a. CD11A, LFA-1, LFA1A)
• Tag / Fusion Protein:
  • tension sensor module

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (unknown if destroyed)
• 3′ cloning site: NotI (unknown if destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7

Resource Information

• Supplemental Documents:
  • aLL-EGFP information

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry