pAAV-synp-F-H2B-GCaMP6f
(Plasmid #102994)

• Purpose: Expresses histone fused GCaMP6f under the synapsin promoter
• Depositing Lab: David Cox
• Publication: Skocek et al Nat Methods. 2018 Jun;15(6):429-432. doi: 10.1038/s41592-018-0008-0. Epub 2018 May 7.

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Backbone

• Vector backbone: pAAV
• Backbone manufacturer: unknown
• Total vector size (bp): 6502
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Growth instructions: Although Stable strains are highly recommended, regular strains (eg, DH5alpha) can be used for better yield.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: H2B-GCaMP6f
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 1662
• Promoter: human synapsin I promoter

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AscI (unknown if destroyed)
• 3′ cloning site: NheI (unknown if destroyed)
• 5′ sequencing primer: GTCGACTCCGGAATAACTTCGTA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry