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Addgene

pHCD1E
(Plasmid #102956)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 102956 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCR2.1-TOPO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 3908
  • Vector type
    Cloning

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CD1E cDNA
  • Alt name
    AJ289111.1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1205
  • Entrez Gene
    CD1E (a.k.a. CD1A, R2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHCD1E was a gift from Gennaro De Libero & Lucia Mori (Addgene plasmid # 102956 ; http://n2t.net/addgene:102956 ; RRID:Addgene_102956)

  • For your References section:

    Fine tuning by human CD1e of lipid-specific immune responses. Facciotti F, Cavallari M, Angenieux C, Garcia-Alles LF, Signorino-Gelo F, Angman L, Gilleron M, Prandi J, Puzo G, Panza L, Xia C, Wang PG, Dellabona P, Casorati G, Porcelli SA, de la Salle H, Mori L, De Libero G. Proc Natl Acad Sci U S A. 2011 Aug 23;108(34):14228-33. doi: 10.1073/pnas.1108809108. Epub 2011 Aug 15. 10.1073/pnas.1108809108 PubMed 21844346
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