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Addgene

pQE 80L Kan T7WT
(Plasmid #102790)

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Item Catalog # Description Quantity Price (USD)
Plasmid 102790 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pQE
  • Backbone manufacturer
    Qiagen
  • Total vector size (bp) 7154
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    T7 RNA polymerase
  • Species
    T7 phage
  • Insert Size (bp)
    2685

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQE 80L Kan T7WT was a gift from Andrew Ellington (Addgene plasmid # 102790 ; http://n2t.net/addgene:102790 ; RRID:Addgene_102790)

  • For your References section:

    Compartmentalized partnered replication for the directed evolution of genetic parts and circuits. Abil Z, Ellefson JW, Gollihar JD, Watkins E, Ellington AD. Nat Protoc. 2017 Dec;12(12):2493-2512. doi: 10.1038/nprot.2017.119. Epub 2017 Nov 9. 10.1038/nprot.2017.119 PubMed 29120463
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