p416TEF-SEC16
(Plasmid
#102676)
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PurposeThe gene SEC16 is driven by the strong promoter TEF. Overexpression of SEC16 increases recombinant protein secretion
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 102676 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonep416TEF
- Total vector size (bp) 12088
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameSEC16
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)6588
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GenBank IDYPL085W
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Entrez GeneSEC16 (a.k.a. YPL085W, LPF1)
- Promoter TEF
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p416TEF-SEC16 was a gift from Jens Nielsen (Addgene plasmid # 102676 ; http://n2t.net/addgene:102676 ; RRID:Addgene_102676) -
For your References section:
Moderate Expression of SEC16 Increases Protein Secretion by Saccharomyces cerevisiae. Bao J, Huang M, Petranovic D, Nielsen J. Appl Environ Microbiol. 2017 Jun 30;83(14). pii: e03400-16. doi: 10.1128/AEM.03400-16. Print 2017 Jul 15. 10.1128/AEM.03400-16 PubMed 28476767