pcDNA3-neo-Strep_Flag_Cterm
(Plasmid
#102645)
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Purpose(Empty Backbone) Epitope tag c-terminus with SBP-Flag moiety
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 102645 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3_neo
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Backbone manufacturerInvitrogen
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
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Tag
/ Fusion Protein
- SBP-Flag, MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREPGGMDYKDDD (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- SBP-Flag, MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREPGGMDYKDDD (C terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
No insert, but contains a single C-terminus Strepavidin binding peptide and Flag epitopes
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-neo-Strep_Flag_Cterm was a gift from Michael E. Wright (Addgene plasmid # 102645 ; http://n2t.net/addgene:102645 ; RRID:Addgene_102645) -
For your References section:
Androgen receptor and chemokine receptors 4 and 7 form a signaling axis to regulate CXCL12-dependent cellular motility. Hsiao JJ, Ng BH, Smits MM, Wang J, Jasavala RJ, Martinez HD, Lee J, Alston JJ, Misonou H, Trimmer JS, Wright ME. BMC Cancer. 2015 Mar 31;15:204. doi: 10.1186/s12885-015-1201-5. 10.1186/s12885-015-1201-5 [pii] PubMed 25884570
