p11d-tRPA-32Ala9
(Plasmid
#102616)
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PurposeExpression of human RPA with an alanine substituted phosphorylation domain (with the following mutations in RPA32 :S8,11-13,23,29,33,39A,T21A).
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 102616 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET11d
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameRPA1
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SpeciesH. sapiens (human)
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Entrez GeneRPA1 (a.k.a. HSSB, MST075, PFBMFT6, REPA1, RF-A, RP-A, RPA70)
- Promoter T7
Cloning Information for Gene/Insert 1
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameRPA3
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Entrez GeneRPA3 (a.k.a. REPA3, RP-A p14)
Gene/Insert 3
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Gene/Insert nameRPA2
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Mutationwith an alanine substituted phosphorylation domain
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Entrez GeneRPA2 (a.k.a. REPA2, RP-A p32, RP-A p34, RPA32)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
nt regions for each gene:
RPA70 5317-7164; RPA32 8413-9226; RPA14 7706-8068
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p11d-tRPA-32Ala9 was a gift from Marc Wold (Addgene plasmid # 102616 ; http://n2t.net/addgene:102616 ; RRID:Addgene_102616)
