Skip to main content
Addgene

pRSF-FGE
(Plasmid #102615)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 102615 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pRSF-Duet
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Formylglycine-generating enzyme
  • Alt name
    NP_215226
  • Alt name
    Rv0712
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PfuI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

FSG gene on PfoI-XhoI fragment from pBAD/myc-hisA Rv0712 (FGE) (Carolyn Bertozzi, Addgene plasmid # 16132) was cloned into pRSF-Duet PfoI-XhoI cut

FGE(rv0712) is nt 4810-5709

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSF-FGE was a gift from Marc Wold (Addgene plasmid # 102615 ; http://n2t.net/addgene:102615 ; RRID:Addgene_102615)

  • For your References section:

    Single-Molecule Analysis of Replication Protein A-DNA Interactions. Bain FE, Fischer LA, Chen R, Wold MS. Methods Enzymol. 2018;600:439-461. doi: 10.1016/bs.mie.2017.11.016. 10.1016/bs.mie.2017.11.016 PubMed 29458769
Related items:
Commonly requested with: