p11d-tRPA(123)
(Plasmid #102613)

• Purpose: Expression of human RPA in E. coli
• Depositing Lab: Marc Wold
• Publication: Henricksen et al J Biol Chem. 1994 Apr 15;269(15):11121-32.

Backbone

• Vector backbone: pET11d
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: RPA1
• Species: H. sapiens (human)
• Entrez Gene: RPA1 (a.k.a. HSSB, MST075, PFBMFT6, REPA1, RF-A, RP-A, RPA70)
• Promoter: T7

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: T7

Gene/Insert 2

• Gene/Insert name: RPA2
• Alt name: RPA32
• Species: H. sapiens (human)
• Entrez Gene: RPA2 (a.k.a. REPA2, RP-A p32, RP-A p34, RPA32)

Gene/Insert 3

• Gene/Insert name: RPA3
• Alt name: RPA14
• Species: H. sapiens (human)
• Entrez Gene: RPA3 (a.k.a. REPA3, RP-A p14)

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry