pSN007
(Plasmid #102440)

• Purpose: For PCR amplification to create paired gRNAs to clone into Golden Gate gRNA expression vectors (e.g. pSB700 Plasmid #64046)
• Depositing Labs: Alejandro Chavez, George Church
• Publication: Nageshwaran et al J Vis Exp. 2018 Oct 2;(140). doi: 10.3791/57998.

Backbone

• Vector backbone: pDONR221
• Backbone size w/o insert (bp): 4762
• Total vector size (bp): 2924
• Modifications to backbone: 7SK paired gRNA insert cloned in
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: 7SK paired gRNA insert
• Species: Synthetic
• Insert Size (bp): 374

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13 F
• 3′ sequencing primer: T7 5'-TAATACGACTCACTATAGGG-3'

Resource Information

• Supplemental Documents:
  • pSN007.gb
  • M13F 7SK fragment
  • M13R 7SK fragment

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry