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Addgene

FUW-RLuc-T2A-PuroR
(Plasmid #102320)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 102320 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    FUW
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Renilla luciferase
  • Species
    Synthetic
  • Insert Size (bp)
    936
  • Promoter Ubc

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (unknown if destroyed)
  • 3′ cloning site EcoR1 (unknown if destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FUW-RLuc-T2A-PuroR was a gift from Rudolf Jaenisch (Addgene plasmid # 102320 ; http://n2t.net/addgene:102320 ; RRID:Addgene_102320)

  • For your References section:

    Histidine catabolism is a major determinant of methotrexate sensitivity. Kanarek N, Keys HR, Cantor JR, Lewis CA, Chan SH, Kunchok T, Abu-Remaileh M, Freinkman E, Schweitzer LD, Sabatini DM. Nature. 2018 Jul 11. pii: 10.1038/s41586-018-0316-7. doi: 10.1038/s41586-018-0316-7. 10.1038/s41586-018-0316-7 PubMed 29995852
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