pET21 gamma-tubulin
(Plasmid #101825)

• Purpose: production of gamma-tubulin in bacteria
• Depositing Labs: Maria Alvarado-Kristensson, Ana Carrera
• Publication: Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. doi: 10.1038/ncb1921. Epub 2009 Aug 2.

Backbone

• Vector backbone: pET21b
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: gamma-tubulin 1
• Alt name: TUBG1
• Species: H. sapiens (human)
• Insert Size (bp): 1347
• Mutation: Whole protein
• GenBank ID:
• Entrez Gene: TUBG1 (a.k.a. CDCBM4, GCP-1, TUBG, TUBGCP1)
• Promoter: T7
• Tag / Fusion Protein:
  • His (C terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: TAA TAC GAC TCA CTA TAG GG

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The TUBG1 was originally cloned by Dr. Jiri Bartek.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry