-
Purpose(Empty Backbone) The plasmid can be either plasmid propogation backbone or expression vector in specific strain
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 101737 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepBR322
-
Backbone manufacturerpCC5.2 ORFB
- Backbone size (bp) 6520
-
Modifications to backboneboth have E. coli and cyanobacteria origin
-
Vector typeBacterial Expression, Synthetic Biology
- Promoter Trc1O
-
Tag
/ Fusion Protein
- eYFP
Growth in Bacteria
-
Bacterial Resistance(s)Spectinomycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberLow Copy
Cloning Information
- Cloning method Gibson Cloning
Resource Information
-
Supplemental Documents
-
Articles Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pSCB-YFP was a gift from Devaki Bhaya (Addgene plasmid # 101737 ; http://n2t.net/addgene:101737 ; RRID:Addgene_101737)
Map uploaded by the depositor.
