p330 VQR
(Plasmid
#101730)
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PurposeExpresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifs
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 101730 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepx330
- Total vector size (bp) 8484
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSpCas9 VQR
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SpeciesS pyogenes
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Insert Size (bp)4101
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MutationVQR (D1135V, R1335Q, and T1337R mutations in Cas9)
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Tags
/ Fusion Proteins
- 3xFLAG-NLS (N terminal on insert)
- NLS (C terminal on insert)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Cas9 VQR sequence cloned from Addgene Plasmid #65771 cloned into px330 Addgene plasmid #42230
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p330 VQR was a gift from Andrew Holland (Addgene plasmid # 101730 ; http://n2t.net/addgene:101730 ; RRID:Addgene_101730) -
For your References section:
Applying the auxin-inducible degradation system for rapid protein depletion in mammalian cells. Lambrus BG, Moyer TC, Holland AJ. Methods Cell Biol. 2018;144:107-135. doi: 10.1016/bs.mcb.2018.03.004. Epub 2018 Apr 5. 10.1016/bs.mcb.2018.03.004 PubMed 29804665
Map uploaded by the depositor.
