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Addgene

px330 VRER
(Plasmid #101729)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 101729 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    px330
  • Total vector size (bp) 8484
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SpCas9 VRER
  • Species
    S pyogenes
  • Insert Size (bp)
    4101
  • Mutation
    VRER (D1135V, G1218R, R1335E, and T1337R mutations in Cas9)
  • Tags / Fusion Proteins
    • 3xFLAG-NLS (N terminal on insert)
    • NLS (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Cas9 VRER sequence cloned from Addgene Plasmid #65773 cloned into px330 Addgene plasmid #42230

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    px330 VRER was a gift from Andrew Holland (Addgene plasmid # 101729 ; http://n2t.net/addgene:101729 ; RRID:Addgene_101729)

  • For your References section:

    Applying the auxin-inducible degradation system for rapid protein depletion in mammalian cells. Lambrus BG, Moyer TC, Holland AJ. Methods Cell Biol. 2018;144:107-135. doi: 10.1016/bs.mcb.2018.03.004. Epub 2018 Apr 5. 10.1016/bs.mcb.2018.03.004 PubMed 29804665
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