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pCDH-EF1-mouse SmyD1-T2A-copGFP
(Plasmid #101157)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 101157 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDH-EF1-MCS-T2A-copGFP
  • Backbone manufacturer
    System Biosciences
  • Total vector size (bp) 8726
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SmyD1
  • Alt name
    Accession # BC076601clone ID30609878 (GE Dharmacon)
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Smyd1 (a.k.a. 4632404M21Rik, Bop, C78565, Zmynd18)
  • Promoter EF1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    SmyD1 synthesized by GE Dharmacon

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDH-EF1-mouse SmyD1-T2A-copGFP was a gift from Martine Roussel (Addgene plasmid # 101157 ; http://n2t.net/addgene:101157 ; RRID:Addgene_101157)

  • For your References section:

    Exome sequencing analysis of murine medulloblastoma models identifies WDR11 as a potential tumor suppressor in Group 3 tumors. Wei L, Murphy BL, Wu G, Parker M, Easton J, Gilbertson RJ, Zhang J, Roussel MF. Oncotarget. 2017 Jul 27. doi: 10.18632/oncotarget.19642. 10.18632/oncotarget.19642 PubMed 28778067
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