pNOC-stacked-aequorin
(Plasmid
#101010)
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Purpose(Empty Backbone) Construct for expression of aequorin, calcium sensitive luciferase reporter and hygromycin resistance gene by a bidirectional promoter
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 101010 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepNOC-ARS-stacked-NeoR-GFP
- Backbone size (bp) 6766
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Vector typeAlgae, Nannochloropsis
- Promoter Ribi
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNOC-stacked-aequorin was a gift from Eva Farre (Addgene plasmid # 101010 ; http://n2t.net/addgene:101010 ; RRID:Addgene_101010) -
For your References section:
Identification of circadian rhythms in Nannochloropsis species using bioluminescence reporter lines. Poliner E, Cummings C, Newton L, Farre EM. Plant J. 2019 Jul;99(1):112-127. doi: 10.1111/tpj.14314. Epub 2019 Apr 19. 10.1111/tpj.14314 PubMed 30883973
Map uploaded by the depositor.
