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Addgene

PBDGTV
(Plasmid #100859)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 100859 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBR322
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The plasmid PBDGTV is a piggyBac transposon based dual directional gene trap vector, in which, the mutagens are two non-selectable gene-trap cassettes in opposite orientations. This vector can be used for selection of mutant clones independent of expression of the target gene. In this vector, a selection marker gene, puroTK, driven by the mouse phosphoglyceratekinase promoter (PGK), allows selection for PB integration into the chromosome using puromycin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PBDGTV was a gift from Yue Huang (Addgene plasmid # 100859 ; http://n2t.net/addgene:100859 ; RRID:Addgene_100859)

  • For your References section:

    Arrayed mutant haploid embryonic stem cell libraries facilitate phenotype-driven genetic screens. Liu G, Wang X, Liu Y, Zhang M, Cai T, Shen Z, Jia Y, Huang Y. Nucleic Acids Res. 2017 Sep 28. doi: 10.1093/nar/gkx857. 10.1093/nar/gkx857 PubMed 29036617
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