pXJ42-p110 CUX1
(Plasmid #100814)

• Purpose: Plasmid vector expressing human p110 CUX1 (amino acids 878-1505 of P39880), with an N-terminal Myc tag and a C-terminal HA tag.
• Depositing Lab: Alain Nepveu
• Publication: Truscott et al Mol Cell Biol. 2008 May;28(10):3127-38. doi: 10.1128/MCB.02089-07. Epub 2008 Mar 17.

Backbone

• Vector backbone: pXJ42
• Modifications to backbone: pXJ42 was obtained from Alan Wildeman. pXJ42 was derived from pXJ40 (Cell 65:551-568, (1991) by changing the polylinker. It contains the human CMV promoter and intron 2 from the rabbit beta-globin gene We used pXJ42 originally because we needed a mammalian vector that did not contain the origin of replication of SV40. In practice, people in the lab found this was the best vector and we continued to use it for other applications.
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: p110 CUX1 (amino acids 878-1505)
• Species: H. sapiens (human)
• Mutation: Contains amino acids 878-1505
• GenBank ID: P39880
• Entrez Gene: CUX1 (a.k.a. CASP, CDP, CDP/Cut, CDP1, COY1, CUTL1, CUX, Clox, Cux/CDP, GDDI, GOLIM6, Nbla10317, p100, p110, p200, p75)
• Tags / Fusion Proteins:
  • Myc (N terminal on insert)
  • HA (C terminal on insert)

Resource Information

• Supplemental Documents:
  • pxj42 p110 CUX1 (878-1505).gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry