CD8-dCherry-FRB
(Plasmid #100739)

• Purpose: To express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis (dark mCherry K70N)
• Depositing Lab: Stephen Royle
• Publication: Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.201702188. doi: 10.1083/jcb.201702188.

Backbone

• Vector backbone: pmCherry-N1
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CD8A
• Alt name: CD8 alpha chain
• Species: H. sapiens (human)
• Mutation: Dark mCherry (K70N) non-fluorescent
• Entrez Gene: CD8A (a.k.a. CD8, CD8alpha, Leu2, p32)
• Promoter: CMV
• Tags / Fusion Proteins:
  • dCherry (C terminal on insert)
  • FRB (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (unknown if destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)
• 5′ sequencing primer: CMV Forward

Resource Information

• Supplemental Documents:
  • CD8-dCherry-FRB.gb
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry