FKBP-beta2(616-951)-GFP ∆CBM
(Plasmid #100728)

• Purpose: To express a clathrin "hook" mutant in mammalian cells to hot-wire endocytosis
• Depositing Lab: Stephen Royle
• Publication: Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.201702188. doi: 10.1083/jcb.201702188.

Backbone

• Vector backbone: pEGFP-N1
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: AP2B1
• Alt name: beta2 adaptin
• Species: H. sapiens (human)
• Insert Size (bp): 1008
• Mutation: Long isoform, deletion of clathrin box motif
• GenBank ID: BC006201 NM_001030006.1
• Entrez Gene: AP2B1 (a.k.a. ADTB2, AP105B, AP2-BETA, CLAPB1)
• Promoter: CMV
• Tags / Fusion Proteins:
  • FKBP (N terminal on insert)
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV Forward

Resource Information

• Supplemental Documents:
  • FKBP-beta2(616-951)-GFP ∆CBM.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry