pUPRT::Floxed DHFR-TS*
(Plasmid
#100606)
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PurposeTgUPRT disruption by knock in with Floxed DHFR-TS*(Pyr-R) cassette with UPRT homology flanks.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 100606 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUC19
- Backbone size w/o insert (bp) 2443
- Total vector size (bp) 8041
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Vector typeToxoplasma expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTgUPRT::Floxed TgDHFR-TS*
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SpeciesToxoplasma gondii
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Insert Size (bp)5598
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MutationPyrimethamine resistance
- Promoter pTgDHFR-TS
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer M13R
- 3′ sequencing primer M13F (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUPRT::Floxed DHFR-TS* was a gift from David Sibley (Addgene plasmid # 100606 ; http://n2t.net/addgene:100606 ; RRID:Addgene_100606)
