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Addgene

pDONOR-tagBFP-PSM-dTOMATO
(Plasmid #100604)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 100604 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC-AMPr based
  • Backbone size (bp) 2898
  • Modifications to backbone
    Insert NSM (negative selection module) and HpaI flanked PSM (ITR positive selection module ITR)
  • Vector type
    Synthetic Biology
  • Selectable markers
    Puromycin
  • Tags / Fusion Proteins
    • tagBFP
    • dTOMATO-T2A-Puro

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDONOR-tagBFP-PSM-dTOMATO was a gift from Jens Schwamborn (Addgene plasmid # 100604 ; http://n2t.net/addgene:100604 ; RRID:Addgene_100604)

  • For your References section:

    FACS-Assisted CRISPR-Cas9 Genome Editing Facilitates Parkinson's Disease Modeling. Arias-Fuenzalida J, Jarazo J, Qing X, Walter J, Gomez-Giro G, Nickels SL, Zaehres H, Scholer HR, Schwamborn JC. Stem Cell Reports. 2017 Nov 14;9(5):1423-1431. doi: 10.1016/j.stemcr.2017.08.026. Epub 2017 Oct 5. 10.1016/j.stemcr.2017.08.026 PubMed 28988985
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