pcDNA3.1-TYMS
(Plasmid #100545)

• Purpose: mammalian expression of thymidylate synthetase
• Depositing Lab: Yuh-Shan Jou
• Publication: Yeh et al Oncotarget. 2017 Sep 8;8(44):77734-77751. doi: 10.18632/oncotarget.20774. eCollection 2017 Sep 29.

Backbone

• Vector backbone: pCDNA3.1+
• Backbone manufacturer: invitrogen
• Backbone size w/o insert (bp): 5428
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: thymidylate synthetase
• Alt name: TS, TMS
• Alt name: HST422, TYMS
• Species: H. sapiens (human)
• Insert Size (bp): 942
• Entrez Gene: TYMS (a.k.a. DKCD, HST422, TMS, TS)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRV (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry