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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 39857 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV6-XL3
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Backbone manufacturerOrigene
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBDNF
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SpeciesM. musculus (mouse)
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Insert Size (bp)750
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GenBank IDX55573
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Entrez GeneBdnf
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (unknown if destroyed)
- 3′ cloning site XbaI (unknown if destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer T3 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV6 BDNF was a gift from Yves-Alain Barde (Addgene plasmid # 39857 ; http://n2t.net/addgene:39857 ; RRID:Addgene_39857) -
For your References section:
Regional distribution of brain-derived neurotrophic factor mRNA in the adult mouse brain. Hofer M, Pagliusi SR, Hohn A, Leibrock J, Barde YA. EMBO J. 1990 Aug;9(8):2459-64. PubMed 2369898