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Addgene

AY22_pgRNA.R5.1
(Plasmid #100294)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 100294 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUCBM21
  • Total vector size (bp) 3046
  • Vector type
    Mammalian Expression, CRISPR, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    gRNA spacer
  • gRNA/shRNA sequence
    Human CCR 5
  • Species
    H. sapiens (human), Synthetic
  • Promoter U6 RNA Pol III promoter human snRNA

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BveI (destroyed during cloning)
  • 3′ cloning site BveI (destroyed during cloning)
  • 5′ sequencing primer M13 Rev
  • 3′ sequencing primer M13 Fwd
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AY22_pgRNA.R5.1 was a gift from Manuel Goncalves (Addgene plasmid # 100294 ; http://n2t.net/addgene:100294 ; RRID:Addgene_100294)

  • For your References section:

    In trans paired nicking triggers seamless genome editing without double-stranded DNA cutting. Chen X, Janssen JM, Liu J, Maggio I, 't Jong AEJ, Mikkers HMM, Goncalves MAFV. Nat Commun. 2017 Sep 22;8(1):657. doi: 10.1038/s41467-017-00687-1. 10.1038/s41467-017-00687-1 PubMed 28939824
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