AY42_pS.Donor.R5
(Plasmid
#100291)
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PurposeCCR5-targeting plasmid
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 100291 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUC57
- Total vector size (bp) 3559
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Vector typeMammalian Expression, CRISPR, Synthetic Biology ; Human Targeting
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name18 bp RFLP (MluI+KspAI)
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SpeciesH. sapiens (human), Synthetic
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Insert Size (bp)818
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AY42_pS.Donor.R5 was a gift from Manuel Goncalves (Addgene plasmid # 100291 ; http://n2t.net/addgene:100291 ; RRID:Addgene_100291) -
For your References section:
In trans paired nicking triggers seamless genome editing without double-stranded DNA cutting. Chen X, Janssen JM, Liu J, Maggio I, 't Jong AEJ, Mikkers HMM, Goncalves MAFV. Nat Commun. 2017 Sep 22;8(1):657. doi: 10.1038/s41467-017-00687-1. 10.1038/s41467-017-00687-1 PubMed 28939824
Map uploaded by the depositor.
