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Addgene

Frb-N-TEV
(Plasmid #100095)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 100095 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pQCXIP
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    N-TEV
  • Alt name
    NIa tobacco etch virus protease
  • Mutation
    N terminal domain
  • Promoter CMV
  • Tags / Fusion Proteins
    • Myc (N terminal on insert)
    • Frb (N terminal on insert)

Cloning Information

  • Cloning method Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Frb-N-TEV was a gift from Jim Wells (Addgene plasmid # 100095 ; http://n2t.net/addgene:100095 ; RRID:Addgene_100095)

  • For your References section:

    Engineered cellular gene-replacement platform for selective and inducible proteolytic profiling. Morgan CW, Diaz JE, Zeitlin SG, Gray DC, Wells JA. Proc Natl Acad Sci U S A. 2015 Jul 7;112(27):8344-9. doi: 10.1073/pnas.1504141112. Epub 2015 Jun 23. 10.1073/pnas.1504141112 PubMed 26106156
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