Yarrowia lipolytica Golden Gate tool kit
(Kit #
1000000167
)
Depositing Lab: Tristan Rossignol
A Golden Gate tool kit containing sixty-four standarized bricks for assembly of up to three transcription units for use in Yarrowia lipolytica
This kit will be sent as bacterial glycerol stocks in 96-well plate format.
Original Publication
A modular Golden Gate toolkit for Yarrowia lipolytica synthetic biology. Larroude M, Park Y-K, Soudier P, Kubiak M, Nicaud J-M, Rossignol T. Microb Biotechnol.2019 Nov;12(6):1249-1259. doi: 10.1111/1751-7915.13427. PubMed PMID: 31148366.
Description
The Yarrowia lipolytica Golden Gate tool kit contains a set of sixty-four standardized bricks that can be used for the one-step assembly of up to three transcription units. The toolbox includes six different selective markers (three auxotrophic markers, two antibiotic-resistance markers, and one metabolic marker), nine different promoters including inducible promoters for each transcription unit, up to six terminators, five pairs of sequences for genome integration and a set of genes coding for fluorescent proteins. The toolbox is made for integrative vector assembly of up to three transcription units in the oleaginous yeast Y. lipolytica.
Schematic drawing of the Golden Gate assembly in Yarrowia lipolytica. Bottom indicates all the bricks available at each position in the three transcription units configuration with letters indicating the 4-nt overhang flanking each brick. Prom: promoter; Ter: terminator; InsertUp and InsertDown: sequences for insertion in the genome.
How to Cite this Kit
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.
For your Materials and Methods section:
“The Yarrowia lipolytica Golden Gate tool kit was a gift from Tristan Rossignol (Addgene kit #1000000167).”
For your Reference section:
A modular Golden Gate toolkit for Yarrowia lipolytica synthetic biology. Larroude M, Park Y-K, Soudier P, Kubiak M, Nicaud, J-M, Rossignol T. Microb Biotechnol.2019 Nov;12(6):1249-1259. doi: 10.1111/1751-7915.13427. PubMed PMID: 31148366.
Yarrowia lipolytica Golden Gate tool kit - #1000000167
- Resistance Color Key
Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.
- Inventory
Searchable and sortable table of all plasmids in kit. The Well column lists the plasmid well location in its plate. The Plasmid column links to a plasmid's individual web page.
- Kit Plate Map
96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.
Resistance Color Key
| Ampicillin | |
| Kanamycin |
Inventory
| Well | Plasmid | Resistance |
|---|---|---|
| A / 1 | GGE0029 | Ampicillin |
| A / 2 | GGE114 | Ampicillin |
| A / 3 | GGE083 | Kanamycin |
| A / 4 | GGE176 | Kanamycin |
| A / 5 | GGE105 | Kanamycin |
| A / 6 | GGE142 | Kanamycin |
| A / 7 | GGE367 | Kanamycin |
| A / 8 | GGE368 | Kanamycin |
| A / 9 | GGE085 | Kanamycin |
| A / 10 | GGE002 | Kanamycin |
| A / 11 | GGE001 | Kanamycin |
| A / 12 | GGE145 | Kanamycin |
| B / 1 | GGE146 | Kanamycin |
| B / 2 | GGE147 | Kanamycin |
| B / 3 | GGE104 | Kanamycin |
| B / 4 | GGE132 | Kanamycin |
| B / 5 | GGE250 | Kanamycin |
| B / 6 | GGE006 | Kanamycin |
| B / 7 | GGE005 | Kanamycin |
| B / 8 | GGE004 | Kanamycin |
| B / 9 | GGE150 | Kanamycin |
| B / 10 | GGE151 | Kanamycin |
| B / 11 | GGE152 | Kanamycin |
| B / 12 | GGE108 | Kanamycin |
| C / 1 | GGE139 | Kanamycin |
| C / 2 | GGE129 | Kanamycin |
| C / 3 | GGE009 | Kanamycin |
| C / 4 | GGE008 | Kanamycin |
| C / 5 | GGE007 | Kanamycin |
| C / 6 | GGE292 | Kanamycin |
| C / 7 | GGE294 | Kanamycin |
| C / 8 | GGE317 | Kanamycin |
| C / 9 | GGE273 | Kanamycin |
| C / 10 | GGE275 | Kanamycin |
| C / 11 | GGE259 | Kanamycin |
| C / 12 | GGE014 | Kanamycin |
| D / 1 | GGE177 | Kanamycin |
| D / 2 | GGE082 | Kanamycin |
| D / 3 | GGE025 | Kanamycin |
| D / 4 | GGE027 | Kanamycin |
| D / 5 | GGE015 | Kanamycin |
| D / 6 | GGE257 | Kanamycin |
| D / 7 | GGE256 | Kanamycin |
| D / 8 | GGE260 | Kanamycin |
| D / 9 | GGE080 | Kanamycin |
| D / 10 | GGE258 | Kanamycin |
| D / 11 | GGE286 | Kanamycin |
| D / 12 | GGE020 | Kanamycin |
| E / 1 | GGE021 | Kanamycin |
| E / 2 | GGE077 | Kanamycin |
| E / 3 | GGE270 | Kanamycin |
| E / 4 | GGE190 | Kanamycin |
| E / 5 | GGE070 | Kanamycin |
| E / 6 | GGE261 | Kanamycin |
| E / 7 | GGE067 | Kanamycin |
| E / 8 | GGE038 | Kanamycin |
| E / 9 | GGE091 | Kanamycin |
| E / 10 | GGE094 | Kanamycin |
| E / 11 | GGE255 | Kanamycin |
| E / 12 | GGE254 | Kanamycin |
| F / 1 | GGE253 | Kanamycin |
| F / 2 | GGE252 | Kanamycin |
| F / 3 | GGE207 | Kanamycin |
| F / 4 | GGE209 | Kanamycin |