GGE015
(Plasmid
#120770)
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PurposePart of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Terminator of gene expression for position 2 (T2_Lip2)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 120770 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCR4Blunt-TOPO
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Vector typeCloning/assembly plasmid
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameT2_Lip2
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Insert Size (bp)130
Cloning Information
- Cloning method TOPO Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GGE015 was a gift from Tristan Rossignol (Addgene plasmid # 120770 ; http://n2t.net/addgene:120770 ; RRID:Addgene_120770) -
For your References section:
A modular Golden Gate toolkit for Yarrowia lipolytica synthetic biology. Larroude M, Park YK, Soudier P, Kubiak M, Nicaud JM, Rossignol T. Microb Biotechnol. 2019 May 31. doi: 10.1111/1751-7915.13427. 10.1111/1751-7915.13427 PubMed 31148366