MoClo Plant Parts II and Infrastructure Kit
(Kit #
1000000135
)
Depositing Lab: Johannes Stuttmann
The MoClo Plant Parts II and Infrastructure Kit contains 95 DNA modules compatible with the Modular Cloning (MoClo) system for hierarchical assembly of plant multigene constructs.
This kit will be sent as bacterial glycerol stocks in 96-well plate format.
Original Publication
Peripheral infrastructure vectors and an extended set of plant parts for the Modular Cloning system. Gantner J., Ordon J., Ilse T., Kretschmer C., Gruetzner R., Lofke C., Dagdas Y., Burstenbinder K., Marillonnet S. and Stuttmann J. PLoS ONE (2018), 13(5), e0197185. May 30; DOI: 10.1371/journal.pone.0197185. PubMed PMID: 29847550.
Description
The MoClo Plant Parts II and Infrastructure Kit contains 95 DNA modules compatible with the Modular Cloning (MoClo) system for hierarchical assembly of plant multigene constructs. Most modules are fully or partially compatible with the GoldenBraid standard.
This kit contains approximately 80 Level 0 modules (phytobricks; e.g. promoters, epitope tags, subcellular localization signals, genes). A further set of Level 0 modules is intended for the assembly of Gateway destination vectors by MoClo. A set of shuttle vectors allows the conversion of MoClo modules containing coding sequences into Gateway entry vectors. Furthermore, the kit contains eight vectors for yeast two hybrid assays or type III secretion system-dependent bacterium-to-plant delivery of proteins compatible with the MoClo system (vectors receive CDS1 or CDS1ns modules). The kit also contains a Golden Gate cloning-compatible TRV-RNA2 vector for virus-induced gene silencing.
Please review Supplemental Tables S2 and S3 from the original publication for more details on using plasmids included in this kit.
The Modular Cloning standard allows Golden Gate assembly of multigene constructs from standardized parts (phytobricks). This kit contains new phytobricks, and also vectors to connect Modular Cloning and Gateway Cloning platforms. Furthermore, the kit contains yeast two hybrid and bacterium-to-plant translocation vectors designed to receive CDS1 and CDS1ns modules of the MoClo system.
How to Cite this Kit
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.
For your Materials and Methods section:
“The MoClo Plant Parts II and Infrastructure Kit was a gift from Johannes Stuttmann (Addgene kit #1000000135).”
For your Reference section:
Peripheral infrastructure vectors and an extended set of plant parts for the Modular Cloning system. Gantner J., Ordon J., Ilse T., Kretschmer C., Gruetzner R., Lofke C., Dagdas Y., Burstenbinder K., Marillonnet S. and Stuttmann J. PLoS ONE (2018), 13, e0197185. 2013 May 30; DOI: 10.1371/journal.pone.0197185. PubMed PMID: 29847550.
MoClo Plant Parts II and Infrastructure Kit - #1000000135
- Resistance Color Key
Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.
- Inventory
Searchable and sortable table of all plasmids in kit. The Well column lists the plasmid well location in its plate. The Plasmid column links to a plasmid's individual web page.
- Kit Plate Map
96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.
Resistance Color Key
| Chloramphenicol and Kanamycin | |
| Chloramphenicol and Spectinomycin | |
| Ampicillin | |
| Kanamycin | |
| Chloramphenicol and Ampicillin | |
| Chloramphenicol and Gentamicin | |
| Spectinomycin |
Inventory
| Well | Plasmid | Resistance |
|---|---|---|
| A / 1 | pJOG130 | Chloramphenicol and Kanamycin |
| A / 2 | pJOG131 | Chloramphenicol and Kanamycin |
| A / 3 | pJOG387 | Chloramphenicol and Spectinomycin |
| A / 4 | pJOG267 | Chloramphenicol and Spectinomycin |
| A / 5 | pJOG562 | Chloramphenicol and Kanamycin |
| A / 6 | pJOG417 | Ampicillin |
| A / 7 | pJOG418 | Kanamycin |
| A / 8 | pCK011 | Chloramphenicol and Ampicillin |
| A / 9 | pCK012 | Chloramphenicol and Kanamycin |
| A / 10 | pCK013 | Chloramphenicol and Gentamicin |
| A / 11 | pCK014 | Chloramphenicol and Gentamicin |
| A / 12 | pCK015 | Chloramphenicol and Gentamicin |
| B / 1 | pCK016 | Chloramphenicol and Gentamicin |
| B / 2 | pTRV2-GG | Spectinomycin |
| B / 3 | pTEI036 | Spectinomycin |
| B / 4 | pTEI042 | Spectinomycin |
| B / 5 | pJOG644 | Spectinomycin |
| B / 6 | pTEI038 | Spectinomycin |
| B / 7 | pJOG640 | Spectinomycin |
| B / 8 | pJOG684 | Spectinomycin |
| B / 9 | pJOG603 | Spectinomycin |
| B / 10 | pTEI044 | Spectinomycin |
| B / 11 | pJOG301 | Spectinomycin |
| B / 12 | pAGM28521 | Spectinomycin |
| C / 1 | pJOG299 | Spectinomycin |
| C / 2 | pJOG298 | Spectinomycin |
| C / 3 | pJOG295 | Spectinomycin |
| C / 4 | pJOG300 | Spectinomycin |
| C / 5 | pJOG025 | Spectinomycin |
| C / 6 | pJOG123 | Spectinomycin |
| C / 7 | pJOG026 | Spectinomycin |
| C / 8 | pJOG124 | Spectinomycin |
| C / 9 | pJOG466 | Spectinomycin |
| C / 10 | pJOG467 | Spectinomycin |
| C / 11 | pJOG010 | Spectinomycin |
| C / 12 | pJOG648 | Spectinomycin |
| D / 1 | pTEI067 | Spectinomycin |
| D / 2 | pTEI068 | Spectinomycin |
| D / 3 | pTEI069 | Spectinomycin |
| D / 4 | pTEI070 | Spectinomycin |
| D / 5 | pTEI071 | Spectinomycin |
| D / 6 | pTEI73 | Spectinomycin |
| D / 7 | pCK019 | Spectinomycin |
| D / 8 | pCK020 | Spectinomycin |
| D / 9 | pCK021 | Spectinomycin |
| D / 10 | pAGM1761 | Spectinomycin |
| D / 11 | pJOG020 | Spectinomycin |
| D / 12 | pJOG575 | Spectinomycin |
| E / 1 | pJOG022 | Spectinomycin |
| E / 2 | pJOG213 | Spectinomycin |
| E / 3 | pJOG004 | Spectinomycin |
| E / 4 | pJOG535 | Spectinomycin |
| E / 5 | pJOG577 | Spectinomycin |
| E / 6 | pJOG638 | Spectinomycin |
| E / 7 | pJOG639 | Spectinomycin |
| E / 8 | pJOG170 | Spectinomycin |
| E / 9 | pJOG176 | Spectinomycin |
| E / 10 | pJOG169 | Spectinomycin |
| E / 11 | pJOG175 | Spectinomycin |
| E / 12 | pJOG168 | Spectinomycin |
| F / 1 | pJOG174 | Spectinomycin |
| F / 2 | pJOG142 | Spectinomycin |
| F / 3 | pJOG143 | Spectinomycin |
| F / 4 | pJOG140 | Spectinomycin |
| F / 5 | pJOG141 | Spectinomycin |
| F / 6 | pJOG824 | Spectinomycin |
| F / 7 | pAGM16456 | Spectinomycin |
| F / 8 | pAGM16467 | Spectinomycin |
| F / 9 | pAGM16418 | Spectinomycin |
| F / 10 | pAGM16429 | Spectinomycin |
| F / 11 | pJOG642 | Spectinomycin |
| F / 12 | pJOG317 | Spectinomycin |
| G / 1 | pJOG331 | Spectinomycin |
| G / 2 | pJOG658 | Spectinomycin |
| G / 3 | pJOG659 | Spectinomycin |
| G / 4 | pJOG643 | Spectinomycin |
| G / 5 | pCK032 | Spectinomycin |
| G / 6 | pTEI037 | Spectinomycin |
| G / 7 | pTEI041 | Spectinomycin |
| G / 8 | pCK025 | Spectinomycin |
| G / 9 | pAGM16841 | Spectinomycin |
| G / 10 | pJOG173 | Spectinomycin |
| G / 11 | pJOG172 | Spectinomycin |
| G / 12 | pJOG171 | Spectinomycin |
| H / 1 | pJOG021 | Spectinomycin |
| H / 2 | pJOG351 | Spectinomycin |
| H / 3 | pJOG031 | Spectinomycin |
| H / 4 | pJOG536 | Spectinomycin |
| H / 5 | pJOG545 | Spectinomycin |
| H / 6 | pCK039 | Spectinomycin |
| H / 7 | pCK038 | Spectinomycin |
| H / 8 | pJOG641 | Spectinomycin |
| H / 9 | pTEI039 | Spectinomycin |
| H / 10 | pCK26 | Spectinomycin |
| H / 11 | pJOG822 | Spectinomycin |
| H / 12 | pJOG587 | Spectinomycin |