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Plasmid#169256PurposeAAV transfer plasmid for neuronal expression of soma-targeted jGCaMP8s.DepositorHas ServiceAAV Retrograde, AAV1, and AAV9InsertSoma-jGCaMP8s
UseAAVTags6xHis-tagExpressionMammalianMutationPromoterAvailable sinceMay 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn1-FLEX-CybSEP2-WPRE
Plasmid#207661PurposeMonitoring neuropeptide releaseDepositorInsertCytochrome b-561 (Cyb561 Mouse)
UseAAVTagsExpressionMutationHistidine 86 and 159 to alanines, inserted 2 X SE…PromoterhSynapsinAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-MMLVgag-3xNES-Cas9
Plasmid#181752PurposeMMLV-Gag fused to 3x NES-SpyCas9 with the TSTLLMENSS cleavage site in between NES and Cas9. For production of virus like particles with Cas9 nuclease RNP cargo.DepositorInsertMMLV-Gag-3xNES-Cas9 (Spy)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-HIFR
Plasmid#192946PurposeLentiviral production of HIF-1alpha reporterDepositorInsertHIF-1alpha reporter
UseLentiviralTagsExpressionMutationPromotermCMVAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-SaCas9-U6-sgSlc17a6
Plasmid#124847PurposeMutagenesis of Slc17a6 with SauCas9DepositorInsertSlc17a6 gRNA (Slc17a6 Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLv-CMV-MyoD-ER(T)
Plasmid#26809DepositorInsertMyoD-ER(T) (Myod1 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-C1-mTrim21
Plasmid#105516PurposeExpression of m-Cherry-tagged wildtype mouse Trim21 in mammalian cellsDepositorInsertTRIM21 (Trim21 Mouse)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-C1-mTrim21ΔRING-Box
Plasmid#105517PurposeExpression of mCherry-tagged mouse Trim21 with a deleted RING-box in mammalian cellsDepositorInsertTRIM21 (Trim21 Mouse)
UseTagsmCherryExpressionMammalianMutationdeletion of RING domain (aa124-462)PromoterCMVAvailable sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-C1-mTrim21ΔPRYSPRY
Plasmid#105518PurposeExpression of mCherry-tagged mouse Trim21 with a deleted PRYSPRY domain in mammalian cellsDepositorInsertTRIM21 (Trim21 Mouse)
UseTagsmCherryExpressionMammalianMutationdeletion of PRYSPRY domain (aa1-281)PromoterCMVAvailable sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV mGAD65(delE1) GFP WPRE SV40pA
Plasmid#177316PurposeTo produce the AAV vector that expresses GFP under the control of the mGAD65 promoter. The mGAD65 promoter has highly specificity to GABAergic interneurons.DepositorInsertmGAD65(delE1) promoter, GABAergic interneurons specific promoter
UseAAVTagsExpressionMutationPromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
FLAG-TRAF6-wt
Plasmid#21624DepositorInsertTNF receptor-associated factor 6 (Traf6 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJuly 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-OSKM
Plasmid#20321PurposeLentiviral plasmid for tet-inducible expression of mouse Oct4, Sox2, Klf4 and Myc for iPS cell generationDepositorUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-mCherry-mTrim21
Plasmid#105522PurposeT7 promotor drives in vitro transcription of mCherry-tagged wildtype mouse TRIM21 mRNADepositorInsertTRIM21 (Trim21 Mouse)
UseIn vitro transcriptionTagsmCherryExpressionMutationPromoterT7Available sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
EF1a-L274GMmMetRS-T2A-mCherry
Plasmid#220800PurposeLentivirus expressing mutant tRNA synthetase for incorporation of noncanonical amino acids in nascent proteins plus mCherry reporter and puro resistanceDepositorInsertMars1 (Mars1 Mouse)
UseLentiviralTags2xFLAG and T2A-mCherryExpressionMutationmutation in MetRS to change aa 274 from L to GPromoterEF1aAvailable sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMK444 (EF1-OsTIR1(F74G) Puro)
Plasmid#214374PurposeEF1-OsTIR1(F74G) (PiggyBac)DepositorInsertEF1-OsTIR1(F74G)
UseTagsExpressionMammalianMutationPromoterEF1 promoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Galpha-q ONE-GO
Plasmid#189738PurposeGPCR/G protein BRET biosensorDepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1 SIRT1-FL
Plasmid#105670PurposeMammalian expression construct of mouse SIRT1 full length isoformDepositorInsertSIRT1 (Sirt1 Mouse)
UseTagsExpressionMammalianMutationSynonymous base changes not affecting the protein…PromoterCMVAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
Talin-TS
Plasmid#83376PurposeFRET probe for measuring tension on Talin moleculeDepositorInsertTalin1 (Tln1 Mouse)
UseRetroviralTagsEGFP-F40-tagRFP (tension sensor module)ExpressionMammalianMutationTension sensor module is inserted between V447 an…PromoterCMVAvailable sinceOct. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pEGFP-ninein
Plasmid#73519PurposeEncodes mouse ninein tagged with an EGFP tag at its N-terminusDepositorInsertninein (Nin Mouse)
UseTagsEGFP tagExpressionMammalianMutationPromoterCMVAvailable sinceApril 29, 2016AvailabilityAcademic Institutions and Nonprofits only