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Addgene

pAAV mGAD65(delE1) GFP WPRE SV40pA
(Plasmid #177316)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 177316 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pAAV
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    mGAD65(delE1) promoter, GABAergic interneurons specific promoter
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2542

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note in Addgene's NGS result the M13 ori is inverted and there are few ambiguous bases in one of the ITRs. ITRs can be difficult to sequence. Additionally an 8bp deletion of a GT repeat was identified in the mGAD65(delE1) promoter compared to the depositor's reference sequence. The depositor confirmed that the plasmid is still functional.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV mGAD65(delE1) GFP WPRE SV40pA was a gift from Hirokazu Hirai (Addgene plasmid # 177316 ; http://n2t.net/addgene:177316 ; RRID:Addgene_177316)
  • For your References section:

    GABAergic neuron-specific whole-brain transduction by AAV-PHP.B incorporated with a new GAD65 promoter. Hoshino C, Konno A, Hosoi N, Kaneko R, Mukai R, Nakai J, Hirai H. Mol Brain. 2021 Feb 15;14(1):33. doi: 10.1186/s13041-021-00746-1. 10.1186/s13041-021-00746-1 PubMed 33588899