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Plasmid#75028PurposemCherry^SEC^3xFlag vector with ccdB markers for cloning homology armsDepositorInsertmCherry^SEC^3xFlag
UseCRISPRTags3xFLAG and C. elegans codon optimized mCherryExpressionWormMutationPromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCFJ782 - HygroR
Plasmid#190933PurposeHygromycin resistance co-injection markerDepositorInsertHygromycin resistance gene
UseTagsExpressionWormMutationPromoterrps-0Available sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCFJ90 - Pmyo-2::mCherry::unc-54utr
Plasmid#19327DepositorArticleInsertPmyo-2::mCherry
UseTagsExpressionWormMutationPromoterAvailable sinceOct. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCZGY3163
Plasmid#101248PurposeGFP11::rps-18, ribosomal protein subunit, bound to C-terminal part of GFP. Shows GFP localized to ribosomes when combined with GFP 1-10.DepositorInsertgfp11::rps-18 (rps-18 Nematode, Synthetic)
UseGatewayTagsGFP11ExpressionMutationPromoterAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCFJ601 - Peft-3 Mos1 transposase
Plasmid#34874PurposeMos1 transposase expression vector for use in creating targeted transgene insertions and targeted deletions in C. elegansDepositorInsertMos1 transpoase
UseWorm targetingTagsExpressionMutationPromotereef-1A.1 (eft-3)Available sinceFeb. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCFJ356 - MCS(cxTi10816, IV)
Plasmid#34871DepositorInsertcxTi10816 targeting
UseWorm targetingTagsExpressionMutationPromoterAvailable sinceFeb. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCFJ150-mCherry(dpiRNA)::ANI-1(AHPH)
Plasmid#107939Purposepie-1p::mCherry(dpiRNA)::ANI-1(AHPH)::pie-1 3'UTR construct used for MosSCI in nematode. mCherry is optimized by removing all piRNA targeting sites to allow germline expression. RhoA biosenser.DepositorInsertmCherry(dpiRNA)::ANI-1(AHPH)
UseTagsExpressionWormMutationPromoterpie-1Available sinceApril 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCFJ104 - Pmyo-3::mCherry::unc-54
Plasmid#19328DepositorArticleInsertPmyo-3::mCherry
UseTagsmCherryExpressionWormMutationPromoterAvailable sinceNov. 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET_SZ1-RGG-GFP-RGG
Plasmid#124937PurposeExpression of SZ1-RGG-GFP-RGGDepositorInsertSYNZIP1-RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDD286 (TagRFP^SEC^3xMyc)
Plasmid#70684PurposeTagRFP-T^SEC^3xMyc vector with ccdB sites for cloning homology arms.DepositorInsertTagRFP-T-C1^SEC^3xFlag
UseCRISPR and Cre/LoxTags3xMyc and C. elegans codon-optimized TagRFP-TExpressionWormMutationPromoterAvailable sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET_RGG-RGG
Plasmid#124941PurposeExpresion of RGG-RGGDepositorInsertRGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET_SZ1-RGG-RGG
Plasmid#124942PurposeExpression of SZ1-RGG-RGGDepositorInsertSYNZIP1-RGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2474 - pEXP[Psmu-2 | cas9(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]
Plasmid#159816PurposeOptimized enzyme for genome editing in C. elegansDepositorInsertpEXP[Psmu-2 | cas9(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHW394 (15xUAS::GFP::let-858 3'UTR)
Plasmid#85584PurposecGAL effector (15xUAS::GFP::let-858 3'UTR) construct for C. elegansDepositorInsert15xUAS-delta pes-10-GFP
UseTagsExpressionWormMutationGFP(S65C) with three introns for expression in C.…Promoter15xUAS-delta pes-10-GFPAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSEM318 - sgRNA mosTI unc-119 single-copy
Plasmid#159822PurposeSingle copy or array insertion by MosTI using split unc-119 selectionDepositorInsertsgRNA mosTI unc-119 single-copy
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
3xHA-miniTurbo_pAS32
Plasmid#118222Purposeexpresses 3xHA-tagged miniTurbo in C. elegans intestineDepositorInsertminiTurbo (BirA mutant)
UseTags3x HA tagExpressionWormMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …Promoterges-1pAvailable sinceJan. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
ChrII_ttTi5605_loxP_myo2_neoR
Plasmid#194056PurposeDual-selection cassette plasmid for knocking-in at the ChrII_ttTi5605 of the C. elegans genomeDepositorInsertttTi5605
UseTagsExpressionWormMutationPromoterNoneAvailable sinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
ChrV_oxTi365_loxP_myo2_neoR
Plasmid#194057PurposeDual-selection cassette plasmid for knocking-in at the ChrV_oxTi365 of the C. elegans genomeDepositorInsertoxTi365
UseTagsExpressionWormMutationPromoterNoneAvailable sinceFeb. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
KG#908
Plasmid#110914PurposeExpresses the plant proteins TIR1 (necessary for Auxin-Inducible Degradation) pan-neuronally in C. elegansDepositorInsertsrab-3 promoter
TIR1 cDNA
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTK73.ChrII_ttTi5605.sgRNA
Plasmid#194058PurposesgRNA targeting ChrII_ttTi5605 locus of the C. elegans genomeDepositorInsertChrII_ttTi5605 sgRNA
UseTagsExpressionWormMutationPromoterU6Available sinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only