Human Genome-Wide CRISPRa-v2 Libraries
(Pooled Libraries #83978, #1000000091)
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Purpose
Version 2 - Human genome-scale CRISPRa library for activation of gene transcription. Available as 5 gRNAs (single half-library) or 10 gRNAs per gene.
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Vector Backbone
pCRISPRia-v2 (Plasmid #84832) - does not express Cas9
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Depositing Labs
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |||
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Pooled Library | 1000000091 |
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1 | $850 | Add to Cart | ||
Pooled Library | 83978 |
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1 | $540 | Add to Cart |
Library Details
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SpeciesHuman
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Genes Targeted18,915
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gRNAs
- #83978: 104,540
- #1000000091: 209,080
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Controls
- #83978: 1,895
- #1000000091: 3,790
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Lentiviral Generation3rd
Library Shipping
Each library is delivered in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.
Pooled Library #83978 will be delivered as a single tube containing a pooled half-library with 5 gRNAs per gene.
Pooled Library #1000000091 will be delivered as two tubes, each containing a pooled half-library with 5 gRNAs per gene (for a total of 10 unique gRNAs per gene).
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Volume∼20 µL
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Concentration25 ng/µL
Resource Information
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Protocols
- Illumina Sequencing Sample Preparation (PDF, 752 KB)
- Liquid Culture Re-Amplification Protocol (PDF, 71 KB)
- Cell Line Creation and Validation Primer (PDF, 125 KB)
- CRISPRi/a Custom Library Cloning Protocol (PDF, 92 KB)
- For more links and protocols, please see the Weismann lab's CRISPRi/a screening materials page.
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Depositor Data
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Terms and Licenses
Academic/Nonprofit TermsIndustry Terms- Not Available to Industry
Trademarks- Zeocin® is an InvivoGen trademark.
Depositor Comments
In addition to SunTag-VP64 constructs (#60903 and #60904), another dCas9-containing construct that can be used with these libraries is dCas9-VPR (#63798).
The data analysis pipeline for analyzing sequencing data is available on GitHub.
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
hCRISPRa-v2 library was a gift from Jonathan Weissman (Addgene ID #1000000091)
hCRISPRa-v2 library was a gift from Jonathan Weissman (Addgene ID #83978) -
For your References section:
Compact and highly active next-generation libraries for CRISPR-mediated gene repression and activation . Horlbeck MA, Gilbert LA, Villalta JE, Adamson B, Pak RA, Chen Y, Fields AP, Park CY, Corn JE, Kampmann M, Weissman JS Elife. 2016 Sep 23;5. pii: e19760. doi: 10.7554/eLife.19760. PubMed 27661255