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Addgene

pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP
(Plasmid #60903)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 60903 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pHR
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas9 dead

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For more information, visit https://valelab4.ucsf.edu/external/research/suntag.html

Note this plasmid was used with the CRISPRa library, as described in Gilbert et al. Cell 2014 PubMed ID: 25307932.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP was a gift from Ron Vale (Addgene plasmid # 60903 ; http://n2t.net/addgene:60903 ; RRID:Addgene_60903)
  • For your References section:

    A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging. Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD. Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. doi: 10.1016/j.cell.2014.09.039. 10.1016/j.cell.2014.09.039 PubMed 25307933