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Cholesterol determines the cytosolic entry and seeded aggregation of tau.

Tuck BJ, Miller LVC, Katsinelos T, Smith AE, Wilson EL, Keeling S, Cheng S, Vaysburd MJ, Knox C, Tredgett L, Metzakopian E, James LC, McEwan WA
Cell Rep. 2022 May 3;39(5):110776. doi: 10.1016/j.celrep.2022.110776. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
187834pAAV-hSyn-eGFP-P2A-LgBiT-NLSExpression of eGFP-P2A-LgBiT-NLS from human synapsin promoter via adeno-associated virus for neuronal expression.
187835pRK172-6xHis-0N4R-P301S-tau-HiBiTExpression of 6xHis-tagged P301S tau of 0N4R isoform with a C-terminal HiBiT tag for bimolecular luminescence with LgBiT.

Antibodies from Article