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Addgene

pAAV-hSyn-eGFP-P2A-LgBiT-NLS
(Plasmid #187834)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187834 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pAAV
  • Backbone size w/o insert (bp) 4527
  • Total vector size (bp) 5850
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    eGFP-P2A-LgBiT-NLS
  • Alt name
    GPLN
  • Insert Size (bp)
    1323
  • Promoter hSyn

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer CTGCCTCAGTCTGCGGTG
  • 3′ sequencing primer TGAAAGCCATACGGGAAGCA
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-hSyn-eGFP-P2A-LgBiT-NLS was a gift from William McEwan (Addgene plasmid # 187834 ; http://n2t.net/addgene:187834 ; RRID:Addgene_187834)
  • For your References section:

    Cholesterol determines the cytosolic entry and seeded aggregation of tau. Tuck BJ, Miller LVC, Katsinelos T, Smith AE, Wilson EL, Keeling S, Cheng S, Vaysburd MJ, Knox C, Tredgett L, Metzakopian E, James LC, McEwan WA. Cell Rep. 2022 May 3;39(5):110776. doi: 10.1016/j.celrep.2022.110776. 10.1016/j.celrep.2022.110776 PubMed 35508140