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Optimising PHBV biopolymer production in haloarchaea via CRISPRi-mediated redirection of carbon flux.

Lin L, Chen J, Mitra R, Gao Q, Cheng F, Xu T, Zuo Z, Xiang H, Han J
Commun Biol. 2021 Aug 25;4(1):1007. doi: 10.1038/s42003-021-02541-z. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
174382pWL502-PAMPlasmids bearing protospacer sequences with functional PAM efficiently triggered CRISPR-mediated defense in cells with corresponding spacer sequence; article demonstrates use in Haloferax mediterranei
174383pWL502-crRNAThe plasmids carrying a mini-CRISPR array were used to express crRNAs; article demonstrates use in Haloferax mediterranei
174384pBM23-pyrF-NTpBM23 contained pyrF and mini-CRISPR.
174385pHFX-cas3UDPlasmids for gene knockout were constructed based on suicide plasmid pHFX
174388pMD18-T-pyrFmodified pMD18-T containing pyrF and its native promoter, Ampr
174390pHFX-B60-EPSUDPlasmids for gene knockout were constructed based on suicide plasmid pHFX-B60
174396pBM23-pyrF-CSpBM23 contained pyrF and mini-CRISPR.

Antibodies from Article